Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MicroRNA-197-3p induces apoptosis in human prolactinoma cells via ERK/EGR1 signaling pathway

Binbin Zhang, Kai Xue, Yanming Zhang

Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin30 0060, China; Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin30 0060, China;

For correspondence:- Yanming Zhang Email: zym1168@sina.com

Accepted: 1 December 2021 Published: 30 December 2021

Citation: Zhang B, Xue K, Zhang Y. MicroRNA-197-3p induces apoptosis in human prolactinoma cells via ERK/EGR1 signaling pathway. Trop J Pharm Res 2021; 20(12):2469-2474 doi: 10.4314/tjpr.v20i12.2

© 2021 The authors.
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Abstract

Purpose: To determine the effect and therapeutic implications of miR-1973p in human prolactinoma cells.

Methods: The expression of miRNA-197-3p was assayed by quantitative real-time polymerase chain reaction (qRT-PCR). Cell counting kit 8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), and annexin V/PI staining assays were used for the determination of cell viability, proliferation and apoptosis, respectively. Protein expressions were assessed by western blotting.

Results: Overexpression of miR-197-3p caused significant (p < 0.05) inhibition of the viability and proliferation of GH3 prolactinoma cells. Furthermore, the colony-forming potential of GH3 cells was significantly reduced by miR-197-3p overexpression. The tumor-suppressive effects of miR-197-3p occurred via initiation of apoptosis which, in turn, was accompanied by up-regulation of Bax and downregulation of Bcl-2 (p < 0.05). Overexpression of miR-197-3p also inhibited ERK/EGR1 signaling pathway in prolactinoma cells.

Conclusion: MiR-197-3p exhibits a tumor suppressive effect in prolactinoma cells. Therefore, it may have therapeutic potential for the management of prolactinoma.

Keywords: Prolactinoma, adenoma, micro-RNA, miR-197-3p, apoptosis, ERK/EGR1 pathway